Table 3 Detection of BDD treponeme phylogroups according to lesion stage. The effect of BDD lesion stage on detection of BDD treponeme phylogroups in post-trimming and post-disinfection hoof knife swab samples (disinfected using water, 2% Virkon®, 2% sodium hypochlorite or 1:100 FAM30®) as measured by both direct and post-culture PCR
Lesion Type | Contact with lesion (Yes/No) | Post-trimming Direct PCR | Post-disinfection Direct PCR | Post-trimming Culture PCR | Post-disinfection Culture PCR |
|---|---|---|---|---|---|
M1(n = 1) | No | 1/1 (100%) | 1/1 (100%) | 0 | 0 |
M2(n = 7) | No | 3/7 (42.9%) | 2/7 (28.6%) | 0 | 0 |
M2(n = 5) | Yes | 5/5 (100%) | 2/5 (40.0%) | 4/5 (80.0%) | 0 |
M3(n = 6) | No | 4/6 (66.7%) | 3/6 (50.0%) | 1/6 (16.7%) | 0 |
M3(n = 4) | Yes | 4/4 (100%) | 3/4 (75.0%) | 3/4 (75.0%) | 0 |
M4(n = 7) | No | 3/7 (42.9%) | 3/7 (42.9%) | 0 | 0 |
M4(n = 94) | Yes | 94/94 (100%) | 29/94 (40.4%) | 34/94 (36.2%) | 0 |
M4.1(n = 1) | No | 1/1 (100%) | 1/1 (100%) | 0 | 0 |
M4.1(n = 9) | Yes | 9/9 (100%) | 4/9 (44.4%) | 6/9 (66.7%) | 0 |
All lesions (n = 22) | No | 12/22 (54.5%) | 10/22 (45.4%) | 1/22 (4.5%) | 0 |
All lesions (n = 111) | Yes | 111/111 (100%) | 38/111 (34.2%) | 47/111 (39.8%) | 0 |