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Table 3 Detection of BDD treponeme phylogroups according to lesion stage. The effect of BDD lesion stage on detection of BDD treponeme phylogroups in post-trimming and post-disinfection hoof knife swab samples (disinfected using water, 2% Virkon®, 2% sodium hypochlorite or 1:100 FAM30®) as measured by both direct and post-culture PCR

From: Removal of bovine digital dermatitis-associated treponemes from hoof knives after foot-trimming: a disinfection field study

Lesion Type Contact with lesion (Yes/No) Post-trimming Direct PCR Post-disinfection Direct PCR Post-trimming Culture PCR Post-disinfection Culture PCR
M1(n = 1) No 1/1 (100%) 1/1 (100%) 0 0
M2(n = 7) No 3/7 (42.9%) 2/7 (28.6%) 0 0
M2(n = 5) Yes 5/5 (100%) 2/5 (40.0%) 4/5 (80.0%) 0
M3(n = 6) No 4/6 (66.7%) 3/6 (50.0%) 1/6 (16.7%) 0
M3(n = 4) Yes 4/4 (100%) 3/4 (75.0%) 3/4 (75.0%) 0
M4(n = 7) No 3/7 (42.9%) 3/7 (42.9%) 0 0
M4(n = 94) Yes 94/94 (100%) 29/94 (40.4%) 34/94 (36.2%) 0
M4.1(n = 1) No 1/1 (100%) 1/1 (100%) 0 0
M4.1(n = 9) Yes 9/9 (100%) 4/9 (44.4%) 6/9 (66.7%) 0
All lesions (n = 22) No 12/22 (54.5%) 10/22 (45.4%) 1/22 (4.5%) 0
All lesions (n = 111) Yes 111/111 (100%) 38/111 (34.2%) 47/111 (39.8%) 0