Fig. 1

Direct PCR results showing disinfectant efficacy (no contact made between hoof knives and BDD lesions). Treponema DNA Positive Samples (%) identified by direct PCR showing efficacy of 2% Virkon® compared to water for disinfection of hoof knife blades for removal of Treponema genus DNA and DNA from three BDD-associated treponeme phylogroups. No contact was made between the hoof knife blades and the BDD lesions (n = 22)