Treatmentb | Mean ELISA – O.D. values of IgA/IgG specific to MG at different ages (d) |
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14 | 21 | 28c | 35 |
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1 | 0.01/0.01 | 0.01/0.41 | 0.41/0.91 | 0.61/1.41 |
2 | 0.01/0.01 | 0.01/0.02 | 0.02/0.02 | 0.32/0.72 |
3 | 0.01/0.01 | 0.21/0.93 | 0.73/1.83 | 1.23/2.43 |
4 | 0.01/0.01 | 0.01/0.02 | 0.01/0.01 | 0.04/0.24 |
5 | 0.01/0.01 | 0.01/0.83 | 0.63/1.93 | 0.85/1.85 |
6 | 0.01/0.01 | 0.01/0.02 | 0.02/0.02 | 0.06/0.06 |
- aVaccination and/or EE administration to broilers at 14 and 21 days of age
- bTRT 1 = Vaccine administration containing H9N2 and MG antigens and challenged; TRT 2 = EE administration only and challenged; TRT 3 = Vaccine administration containing H9N2, MG, EE, and challenged; TRT 4 = Only challenged (positive controls); TRT 5 = Vaccine administration containing H9N2, MG, EE, and deprived of challenge; TRT 6 = Deprived of antigens and EE, and of challenge (Negative Controls)
- cChallenge at 28 days of age with 4HA units/50 μl of each of H9N2 and MG
- 1-6Means in numerators of a column followed by different Arabic numerical -superscripts are significantly different (P < 0.05); similarly, means in denominators of a column followed by different Arabic numerical - superscripts are also significantly different (P < 0.05)