Treatmentb | Mean ELISA – O.D. values of IgA/IgG specific to H9N2 at different ages (d) |
|---|
14 | 21 | 28c | 35 |
|---|
1 | 0.01/0.01 | 0.21/0.61 | 0.51/1.21 | 0.81/1.81 |
2 | 0.01/0.01 | 0.02/0.02 | 0.02/0.02 | 0.32/0.92 |
3 | 0.01/0.01 | 0.63/1.53 | 0.93/2.33 | 1.53/2.93 |
4 | 0.01/0.01 | 0.01/0.02 | 0.01/0.01 | 0.14/0.44 |
5 | 0.01/0.01 | 0.53/1.63 | 0.73/2.23 | 1.05/2.15 |
6 | 0.01/0.01 | 0.02/0.02 | 0.02/0.02 | 0.04/0.06 |
- aVaccination and/or EE administration to broilers at 14 and 21 days of age
- bTRT 1 = Vaccine administration containing H9N2 and MG antigens and challenged; TRT 2 = EE administration only and challenged; TRT 3 = Vaccine administration containing H9N2, MG, EE, and challenged; TRT 4 = Only challenged (positive controls); TRT 5 = Vaccine administration containing H9N2, MG, EE, and deprived of challenge; TRT 6 = Deprived of antigens and EE, and of challenge (Negative Controls)
- cChallenge at 28 days of age with 4HA units/50 μl of each of H9N2 and MG
- 1-6Means in numerators of a column followed by different Arabic numerical - superscripts are significantly different (P < 0.05); similarly, means in denominators of a column followed by different Arabic - numerical superscripts are also significantly different (P < 0.05)
