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Table 1 Experimental Design of differently treated broilers

From: Evaluation in broilers of aerosolized nanoparticles vaccine encapsulating imuno-stimulant and antigens of avian influenza virus/Mycoplasma gallisepticum

Treatmenta

Aerosolization by liposomal nanoparticles encapsulating the below materialsb

Challengec

Solubilized antigens of H9N2 and MG

Echinacea extract (EE)

1

+

–

+

2

–

+

+

3

+

+

+

4

–

–

+

5

+

+

–

6

–

–

–

  1. aEach treatment had three replicate pens, with 20 birds/pen
  2. bAerosolization of 2 ml/bird at a distance of 20 cm from nostrils, and at the age of 14 and 21 d
  3. cBivalent intra-tracheal challenge at 28 days of age with 0.5 ml/bird of each of H9N2 and MG, at individual concentration of 4 HA units/50 μl