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Table 3 Serological tests and methods used, and results of tests performed in marsh deer (Blastocerus dichotomus) pathogens in Argentina

From: A participatory surveillance of marsh deer (Blastocerus dichotomus) morbidity and mortality in Argentina: first results

Pathogen

Test procedure (positive titre)

Number positive/number tested

Bluetongue virus

AGID (1:4)

0/29

Infectious bovine rhinotracheitis (Bovine herpesvirus)

ELISA

0/29

Bovine viral diarrhoea virus

ELISA

0/29

Brucellosis

BPA /ROSEBEN /2ME (1:100) / SAT (1:100)

1/29

Foot-and-mouth disease virus

VIAA

0/29

Johne’s disease (Mycobacterium avium subsp. paratuberculosis)

AGID

0/29

Leptospira interrogans (11 serovars) a

MAT (1:50)

2/29

Bovine leucosis

AGID

0/29

Q Fever b

Indirect multi-species ELISA

0/29

Chlamydial abortion c

Indirect multi-species ELISA

0/29

Vesicular Stomatitis Virus Indiana and New Jersey Serotype

ELISA-LP (liquid phase)

0/29

  1. References: AGID: agar gel Immunodiffusion; ELISA: enzyme-linked immunodiffusion assay; BPA: buffered plate antigen test; ROSEBEN: rose bengal test; 2ME: 2-mercaptoethanol test; SAT: tube agglutination test; VIAA: virus infection-associated antigen; MAT: microagglutination test. Tests performed at National Service of Agri-food health and quality (SENASA)
  2. aLeptospira interrogans serovars ballum, castellonis, canicola, grippotyphosa, icterohaemorrhagiae, copenhageni, pomona, pyrogenes, sejroe, wolffi, tarassovi
  3. bID Screen® Q Fever Indirect ELISA Multi-species kit (ID.vet, France)
  4. cID Screen® Chlamydophila abortus Indirect ELISA Multi-species kit (ID.vet, France)