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Table 3 Conventional RT-PCR assay and real-time PCR assay detect thirty-nine samples respectively from different affected organs at 24, 48 and 72 h

From: A TaqMan-based real-time PCR assay for specific detection of novel duck reovirus in China

  

Conventional RT-PCR assay

Real-time PCR assay

No

Samples

Number of positive/ Number of samples

Positive rate(%)

Number of positive/ Number of samples

Positive rate(%)

viral copy numbers

1

Heart (24hpi)

0/3

0

0/3

0

Neg.

Neg.

Neg.

2

Liver (24hpi)

0/3

0

0/3

0

Neg.

Neg.

Neg.

3

Spleen (24hpi)

2/3

66.7

2/3

66.7

102.8

Neg.

101.7

4

Lung (24hpi)

3/3

100

3/3

100

103.4

104.2

104.9

5

Kidney(24hpi)

0/3

0

0/3

0

Neg.

Neg.

Neg.

6

Pancreas(24hpi)

0/3

0

0/3

0

Neg.

Neg.

Neg.

7

Stomach(24hpi)

0/3

0

1/3

33.3

Neg.

Neg.

100.9

8

Brain (24hpi)

3/3

100

3/3

100

102.7

103.4

102.0

9

Intestinal(24hpi)

0/3

0

0/3

0

Neg.

Neg.

Neg.

10

Bursa(24hpi)

1/3

33.3

2/3

66.7

Neg.

102.9

102.4

11

Thymus (24hpi)

0/3

0

2/3

66.7

103.4

102.5

Neg.

12

Stool(24hpi)

0/3

0

0/3

0

Neg.

Neg.

Neg.

13

Serum (24hpi)

2/3

66.7

2/3

66.7

102.8

103.9

Neg.

14

Heart (48hpi)

2/3

66.7

3/3

100

101.3

102.4

102.6

15

Liver (48hpi)

1/3

33.3

2/3

66.7

102.9

102.1

Neg.

16

Spleen (48hpi)

3/3

100

3/3

100

104.2

103.3

102.5

17

Lung (48hpi)

3/3

100

3/3

100

103.7

102.1

103.0

18

Kidney(48hpi)

1/3

33.3

2/3

66.7

102.6

102.5

Neg.

19

Pancreas(48hpi)

2/3

66.7

2/3

66.7

102.2

102.8

Neg.

20

Stomach(48hpi)

3/3

100

3/3

100

102.3

102.3

102.5

21

Brain (48hpi)

3/3

100

3 /3

100

102.2

103.2

103.1

22

Intestinal(48hpi)

1/3

33.3

2/3

66.7

105.8

Neg.

105.8

23

Bursa(48hpi)

3/3

100

3/3

100

102.3

103.9

102.8

24

Thymus (48hpi)

1/3

33.3

2/3

66.7

Neg.

102.6

103.0

25

Stool(48hpi)

3/3

100

3/3

100

104.7

104.5

104.8

26

Serum (48hpi)

3/3

100

3/3

100

103.5

103.5

103.4

27

Heart (72hpi)

2/3

66.7

3/3

100

102.6

102.8

102.9

28

Liver (72hpi)

3/3

100

3/3

100

102.6

102.5

101.8

29

Spleen (72hpi)

3/3

100

3/3

100

105.4

107.4

105.2

30

Lung (72hpi)

3/3

100

3/3

100

104.9

105.6

104.2

31

Kidney(72hpi)

3/3

100

3/3

100

102.0

103.8

103.2

32

Pancreas(72hpi)

3/3

100

3/3

100

102.4

101.9

103.2

33

Stomach(72hpi)

3/3

100

3/3

100

102.5

103.5

103.1

34

Brain (72hpi)

3/3

100

3/3

100

102.4

104.6

103.1

35

Intestinal(72hpi)

3/3

100

3/3

100

105.4

106.8

106.2

36

Bursa(72hpi)

3/3

100

3/3

100

105.3

106.0

106.3

37

Thymus (72hpi)

3/3

100

3/3

100

102.9

102.9

102.6

38

Stool(72hpi)

3/3

100

3/3

100

106.5

106.2

104.7

39

Serum (72hpi)

3/3

100

3/3

100

103.9

103.9

104.0