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Table 3 Conventional RT-PCR assay and real-time PCR assay detect thirty-nine samples respectively from different affected organs at 24, 48 and 72 h

From: A TaqMan-based real-time PCR assay for specific detection of novel duck reovirus in China

   Conventional RT-PCR assay Real-time PCR assay
No Samples Number of positive/ Number of samples Positive rate(%) Number of positive/ Number of samples Positive rate(%) viral copy numbers
1 Heart (24hpi) 0/3 0 0/3 0 Neg. Neg. Neg.
2 Liver (24hpi) 0/3 0 0/3 0 Neg. Neg. Neg.
3 Spleen (24hpi) 2/3 66.7 2/3 66.7 102.8 Neg. 101.7
4 Lung (24hpi) 3/3 100 3/3 100 103.4 104.2 104.9
5 Kidney(24hpi) 0/3 0 0/3 0 Neg. Neg. Neg.
6 Pancreas(24hpi) 0/3 0 0/3 0 Neg. Neg. Neg.
7 Stomach(24hpi) 0/3 0 1/3 33.3 Neg. Neg. 100.9
8 Brain (24hpi) 3/3 100 3/3 100 102.7 103.4 102.0
9 Intestinal(24hpi) 0/3 0 0/3 0 Neg. Neg. Neg.
10 Bursa(24hpi) 1/3 33.3 2/3 66.7 Neg. 102.9 102.4
11 Thymus (24hpi) 0/3 0 2/3 66.7 103.4 102.5 Neg.
12 Stool(24hpi) 0/3 0 0/3 0 Neg. Neg. Neg.
13 Serum (24hpi) 2/3 66.7 2/3 66.7 102.8 103.9 Neg.
14 Heart (48hpi) 2/3 66.7 3/3 100 101.3 102.4 102.6
15 Liver (48hpi) 1/3 33.3 2/3 66.7 102.9 102.1 Neg.
16 Spleen (48hpi) 3/3 100 3/3 100 104.2 103.3 102.5
17 Lung (48hpi) 3/3 100 3/3 100 103.7 102.1 103.0
18 Kidney(48hpi) 1/3 33.3 2/3 66.7 102.6 102.5 Neg.
19 Pancreas(48hpi) 2/3 66.7 2/3 66.7 102.2 102.8 Neg.
20 Stomach(48hpi) 3/3 100 3/3 100 102.3 102.3 102.5
21 Brain (48hpi) 3/3 100 3 /3 100 102.2 103.2 103.1
22 Intestinal(48hpi) 1/3 33.3 2/3 66.7 105.8 Neg. 105.8
23 Bursa(48hpi) 3/3 100 3/3 100 102.3 103.9 102.8
24 Thymus (48hpi) 1/3 33.3 2/3 66.7 Neg. 102.6 103.0
25 Stool(48hpi) 3/3 100 3/3 100 104.7 104.5 104.8
26 Serum (48hpi) 3/3 100 3/3 100 103.5 103.5 103.4
27 Heart (72hpi) 2/3 66.7 3/3 100 102.6 102.8 102.9
28 Liver (72hpi) 3/3 100 3/3 100 102.6 102.5 101.8
29 Spleen (72hpi) 3/3 100 3/3 100 105.4 107.4 105.2
30 Lung (72hpi) 3/3 100 3/3 100 104.9 105.6 104.2
31 Kidney(72hpi) 3/3 100 3/3 100 102.0 103.8 103.2
32 Pancreas(72hpi) 3/3 100 3/3 100 102.4 101.9 103.2
33 Stomach(72hpi) 3/3 100 3/3 100 102.5 103.5 103.1
34 Brain (72hpi) 3/3 100 3/3 100 102.4 104.6 103.1
35 Intestinal(72hpi) 3/3 100 3/3 100 105.4 106.8 106.2
36 Bursa(72hpi) 3/3 100 3/3 100 105.3 106.0 106.3
37 Thymus (72hpi) 3/3 100 3/3 100 102.9 102.9 102.6
38 Stool(72hpi) 3/3 100 3/3 100 106.5 106.2 104.7
39 Serum (72hpi) 3/3 100 3/3 100 103.9 103.9 104.0