From: A TaqMan-based real-time PCR assay for specific detection of novel duck reovirus in China
Copies of standard plasmid DNA | Intra-assay variability of Ct values | Interassay variability of Ct values | ||||||
---|---|---|---|---|---|---|---|---|
Proportion of positive Samplesa | Ct | Proportion of positive Samples* | Ct | |||||
Mean | SD | CV (%) | Mean | SD | CV (%) | |||
108 | 1.00 | 15.04 | 0.03 | 0.20 | 1.00 | 15.20 | 0.20 | 1.32 |
107 | 1.00 | 17.47 | 0.06 | 0.34 | 1.00 | 17.69 | 0.17 | 0.96 |
106 | 1.00 | 20.72 | 0.23 | 1.11 | 1.00 | 20.50 | 0.10 | 0.49 |
105 | 1.00 | 23.94 | 0.13 | 0.54 | 1.00 | 23.78 | 0.28 | 1.18 |
104 | 1.00 | 27.11 | 0.02 | 0.07 | 1.00 | 27.85 | 0.78 | 2.80 |
103 | 1.00 | 30.79 | 0.45 | 1.46 | 1.00 | 30.91 | 0.90 | 2.91 |
102 | 1.00 | 34.32 | 0.13 | 0.38 | 1.00 | 34.15 | 0.54 | 1.58 |
10 | 1.00 | 37.84 | 0.56 | 1.48 | 1.00 | 37.24 | 0.35 | 0.94 |