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Table 2 Intra- and inter- assay variability of Ct values of assay in detection of NDRV

From: A TaqMan-based real-time PCR assay for specific detection of novel duck reovirus in China

Copies of standard plasmid DNA Intra-assay variability of Ct values Interassay variability of Ct values
Proportion of positive Samplesa Ct Proportion of positive Samples* Ct
Mean SD CV (%) Mean SD CV (%)
108 1.00 15.04 0.03 0.20 1.00 15.20 0.20 1.32
107 1.00 17.47 0.06 0.34 1.00 17.69 0.17 0.96
106 1.00 20.72 0.23 1.11 1.00 20.50 0.10 0.49
105 1.00 23.94 0.13 0.54 1.00 23.78 0.28 1.18
104 1.00 27.11 0.02 0.07 1.00 27.85 0.78 2.80
103 1.00 30.79 0.45 1.46 1.00 30.91 0.90 2.91
102 1.00 34.32 0.13 0.38 1.00 34.15 0.54 1.58
10 1.00 37.84 0.56 1.48 1.00 37.24 0.35 0.94
  1. aProportion of positive = positive samples/total tested samples (n = 3)