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Table 2 Intra- and inter- assay variability of Ct values of assay in detection of NDRV

From: A TaqMan-based real-time PCR assay for specific detection of novel duck reovirus in China

Copies of standard plasmid DNA

Intra-assay variability of Ct values

Interassay variability of Ct values

Proportion of positive Samplesa

Ct

Proportion of positive Samples*

Ct

Mean

SD

CV (%)

Mean

SD

CV (%)

108

1.00

15.04

0.03

0.20

1.00

15.20

0.20

1.32

107

1.00

17.47

0.06

0.34

1.00

17.69

0.17

0.96

106

1.00

20.72

0.23

1.11

1.00

20.50

0.10

0.49

105

1.00

23.94

0.13

0.54

1.00

23.78

0.28

1.18

104

1.00

27.11

0.02

0.07

1.00

27.85

0.78

2.80

103

1.00

30.79

0.45

1.46

1.00

30.91

0.90

2.91

102

1.00

34.32

0.13

0.38

1.00

34.15

0.54

1.58

10

1.00

37.84

0.56

1.48

1.00

37.24

0.35

0.94

  1. aProportion of positive = positive samples/total tested samples (n = 3)