Table 1 Primers and probe for NDRV detection used in this study
From: A TaqMan-based real-time PCR assay for specific detection of novel duck reovirus in China
RT-PCR | Oligo | Sequence (5′-3′) | Length (bp) | Positions (Segment) |
|---|---|---|---|---|
Realtime | Forward primer | CCCGGATTCTCGATGAATGGT | 21 | 958–978(S2) |
Probe | FAM-AACGCCTGTGCACGAGCTGAAC-3′-TAMRAa | 22 | 981–1022(S2) | |
Reverse primer | CGACCCACTGCTGGATACAAG | 21 | 1022–1042(S2) | |
σC full-length | Forward primer | ATGGATCGCAACGAGGTGATAC | 22 | 571–592(S1) |
Reverse primer | CTAGCCCGTGGCGACGGT | 18 | 1519–1536(S1) | |
σC conventional | Forward primer | TGAGACGCCTGACTACGATT | 20 | 707–726(S1) |
Reverse primer | ATGCTTGGAGTGAGACGACT | 20 | 1056–1075(S1) |