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Fig. 3 | BMC Veterinary Research

Fig. 3

From: A TaqMan-based real-time PCR assay for specific detection of novel duck reovirus in China

Fig. 3

A. Standard curve of the real-time PCR. The triplicate standard curve plots indicate a linear correlation between the Log of the copy number and the CT. The logarithm values (log C) of the detected concentrations of the N-DRV DNA standards (X axis) ranged from 1.0 × 108 to 1.0 × 102 copies/μL, and used the corresponding Threshold cycle (CT value) of each reaction tube fluorescent signal approaching the set threshold (Y axis) of the amplification to perform linear regression. Three replicates were tested for each dilution. B1. Sensitivity of real-time PCR assay for N-DRV detection. B2. Sensitivity of conventional PCR assay for N-DRV detection. M: DL2000 DNA Marker. NC: Nuclease-free water

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