Table 2 Antibodies used for ovine pulmonary tissues immunohistochemistry
Specificity of antibody | Clone | Provider | Dilution | Positive control |
|---|---|---|---|---|
JSRV-MA | – | Prof. Massimo Palmarini | 1/1500 | OPA classical Tumour, PCR+ |
Multi-cytokeratin | AE1/AE3 | LeicaBiosystems Newcastle Ltd | Ready to use | Bronchial epithelium |
Vimentin | clone SRL33 | LeicaBiosystems Newcastle Ltd | Ready to use | Endothelial cells |
TTF-1 | 8G7G3/1 | Cell Marque Milipore Sigma | 1/200 | Type II pneumocytes, bronchioles |
Desmin | DE-R-11 | LeicaBiosystems Newcastle Ltd | Ready to use | Smooth muscle |
SMA | 1A4 | Abcam | 1/200 | Smooth muscle |
S100 | Polyclonal | LeicaBiosystems Newcastle Ltd | Ready to use | Bronchial wall cartillage |
Ki-67 | MM1 | LeicaBiosystems Newcastle Ltd | Ready to use | Lymphoid tissue (BALT) |