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Fig. 4 | BMC Veterinary Research

Fig. 4

From: Exogenous Jaagsiekte Sheep Retrovirus type 2 (exJSRV2) related to ovine pulmonary adenocarcinoma (OPA) in Romania: prevalence, anatomical forms, pathological description, immunophenotyping and virus identification

Fig. 4

JSRV detection from neoplastic tissues. a and a1 Immunocytochemistry showing intracytoplasmic JSRV-MA expression within the neoplastic epithelial cells. DAB and hematoxylin counterstain; B Diffuse expression of the JSRV-MA marker by neoplastic epithelial cells forming tubular structures; c Expression of the JSRV-MA marker by MGs and epithelial tumor. Left inset: Cell cytoplasm of MGs (white arrow) is strongly labelled with JSRV-MA antibody. Right inset: Neoplastic epithelial cells have diffuse cytoplasmic labelling for JSRV-MA (bue arrow). DAB and hematoxylin counterstain; d JSRV-infected type 2 pneumocytes. Note the presence of intracytoplasmic lamellar body (white arrow) and aggregates and solitary intracytoplasmic viral particles (grey arrow). TEM. Bar, 1 μm. Inset: Detail of a viral replication site showing specific virions compatible with JSRV (white arrow). TEM. Bar, 100 nm; e Electrophoresis profile of the amplified DNA fragments (128 bp) evidencing the presence of proviral Jaagsiekte DNA in ovine pulmonary adenocarcinoma (OPA). L - DNA ladder; PCR positive (+) samples; PCR negative (−) samples; M1 and M2 control samples amplified from healthy ovine lung tissue; NTC - no template control (negative control); f Phylogenetic analysis of different JSRV strains and other retroviruses based on the LTR region. The Romanian strains of exJSRV (JSRV-RO) (GenBank MT809678.1) showed homology at nucleotide level of 97% with UK strain (GenBank AF105220.1), and 83% with the South African strain (GenBank M80216)

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