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Fig. 3 | BMC Veterinary Research

Fig. 3

From: Effects of crowding on the three main proteolytic mechanisms of skeletal muscle in rainbow trout (Oncorhynchus mykiss)

Fig. 3

Effect of high density-induced stress on the ubiquitin-proteasome system. a Relative expression (fold change) of key genes of the ubiquitin proteasome system, atrogin1 and murf1, normalized with fau and β-actin. b Representative Western Blot of Ubiquitinated proteins. Coomassie blue-stained loading control proteins. Fold change of ubiquitinated proteins/free ubiquitin ratio. Results are shown as the means ± SEM (n = 5 per group). LD, low stocking density; HD, high stocking density. Significant differences are indicated with a line and an asterisk (* = P < 0.05; ** = P < 0.01; **** = P < 0.0001)

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