Table 2 Primer sequence used for gene expression analysis
From: Thymol modulates the endocannabinoid system and gut chemosensing of weaning pigs
Gene | Primer sequence (F and R) 5′ → 3′ | Product length (bp) | Accession N. | Reference |
|---|---|---|---|---|
CB1 | F: TTCCCCACTTCTTTTCCGCC R: GGGAGTCCCTTCGCATCC | 208 | XM_013992672.2 | Present study |
CB2 | F: TTTATAGCCTGGCCTCCCCT R: TTTTCCCGTCTGCCTCTGTC | 240 | XM_021095530.1 | [50] |
FAAH | F: TGCCACCGTGCAAGAAAATG R: CCACTGCCCTAACAACGACT | 234 | XM_013999418.2 | [50] |
DGL-α | F: GAAACCAAACACGCCTCCAC R: CAACCCAGCAGCAAAGGAAC | 211 | XM_021082924.1 | [50] |
DGL-β | F: TTTGTAATCCCGGACCACGG R: GACCTGCCGAGGAATACGGA | 255 | XM_021086077.1 | Present study |
TRPV1 | F: TCACCAACAAGAAGGGGCTC R: GGATAGGTGCCTGCACTCAG | 116 | XM_005669121.1 | [51] |
OR1G1 | F: CTTGGTTTGTGTGCTCTGCC R: GAAAAGGCTTTCCGCTTCCC | 96 | XM_013990010.1 | Present study |
INF-γ | F: GGCCATTCAAAGGAGCATGGATGT R: TGAGTTCACTGATGGCTTTGCGCT | 149 | NM_213948.1 | [52] |
TNF-α | F: GCCCACGTTGTAGCCAATGTCAAA R: GTTGTCTTTCAGCTTCACGCCGTT | 99 | NM_214022.1 | [52] |
RPL35 | F: AACCAGACCCAGAAAGAGAAC R: TTCCGCTGCTGCTTCTTG | 146 | NM_214326.2 | [53] |