Fig. 1

Gene expression of cannabinoid receptors in duodenal and ileal mucosa of piglets. Data are expressed as means (n = 8) and S.E.M. represented by vertical bars. ^{a, b} Values with different superscripts differ significantly at P < 0.05. T1 = basal diet; T2 = basal diet + 25.5 mg of thymol/kg feed; T3 = basal diet + 51 mg of thymol/kg feed; T4 = basal diet + 153 mg of thymol/kg feed; T5 = basal diet + 510 mg of thymol/kg feed (Vetagro SpA, Reggio Emilia, Italy). A modification of the 2^–ΔΔCT method was used to analyze the relative expression (fold changes), calculated relative to the control group (control; Livak and Schmittgen, 2001 [21]). CB1 = cannabinoid receptor 1; CB2 = cannabinoid receptor 2