TY - JOUR AU - Toschi, Andrea AU - Tugnoli, Benedetta AU - Rossi, Barbara AU - Piva, Andrea AU - Grilli, Ester PY - 2020 DA - 2020/08/12 TI - Thymol modulates the endocannabinoid system and gut chemosensing of weaning pigs JO - BMC Veterinary Research SP - 289 VL - 16 IS - 1 AB - The recent identification of the endocannabinoid system in the gastrointestinal tract suggests a role in controlling intestinal inflammation. In addition, the gut chemosensing system has therapeutic applications in the treatment of gastrointestinal diseases and inflammation due to the presence of a large variety of receptors. The purposes of this study were to investigate the presence of markers of the endocannabinoid system and the chemosensing system in the pig gut and, second, to determine if thymol modulates these markers. One hundred sixty 28-day-old piglets were allocated into one of 5 treatment groups (n = 32 per treatment): T1 (control), T2 (25.5 mg thymol/kg feed), T3 (51 mg thymol/kg feed), T4 (153 mg thymol/kg feed), and T5 (510 mg thymol/kg feed). After 14 days of treatment, piglets were sacrificed (n = 8), and then duodenal and ileal mucosal scrapings were collected. Gene expression of cannabinoid receptors (CB1 and CB2), transient receptor potential vanilloid 1 (TRPV1), the olfactory receptor OR1G1, diacylglycerol lipases (DGL-α and DGL-β), fatty acid amine hydrolase (FAAH), and cytokines was measured, and ELISAs of pro-inflammatory cytokines levels were performed. SN - 1746-6148 UR - https://doi.org/10.1186/s12917-020-02516-y DO - 10.1186/s12917-020-02516-y ID - Toschi2020 ER -