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Table 1  Histological and molecular findings of herpesvirus infection in 14 stranded dolphins

From: Alpha- and gammaherpesviruses in stranded striped dolphins (Stenella coeruleoalba) from Spain: first molecular detection of gammaherpesvirus infection in central nervous system of odontocetes

ID

Sex

Main histopathological findings

Tissues tested for HV

6

M

Systemic congestion, severe; Lymphoplasmacytic and suppurative balanitis, mild; Granulomatous and eosinophilic lymphadenitis, mild; Lymphoplasmacytic cholangitis, mild

Cerebellum, spinal cord (A, MN699008), lung, liver, spleen, kidney, skin, pharyngeal tonsils, tracheobronchial lymph node, pre-scapular lymph node, mesenteric lymph node, penis (G, MN698989)

7

M

Systemic congestion, severe; Meningeal hemorrhage and congestion; Lymphoplasmacytic encephalitis, minimal; Lymphoplasmacytic and suppurative balanitis, mild; Lymphoplasmacytic cholangitis, moderate; Polysaccharide myocyte inclusions

Cerebrum (A, MN699009), cerebellum, spinal cord, lung, liver, spleen, kidney, skin, pharyngeal tonsils, tracheobronchial lymph node, pre-scapular lymph node, mesenteric lymph node, penis (G, MN698990)

8

Fa

Systemic congestion, severe; Lymphoplasmacytic placentitis, minimal; Granulomatous cholangiohepatitis with intralesional trematodes, moderate; Granulomatous and eosinophilic lymphadenitis, moderate; Poxvirus-like dermatitis, mild

Cerebrum, cerebellum, spinal cord, lung, liver, spleen, kidney, skin, pharyngeal tonsils, tracheobronchial lymph node (MT770752), pre-scapular lymph node, mesenteric lymph node.

9

F

Lymphoplasmacytic encephalitis, mild; Systemic congestion and microhemorrhages, severe; suppurative endometritis, mild; Granulomatous and eosinophilic lymphadentitis, moderate

Cerebrum, cerebellum, spinal cord, lung, liver, spleen, kidney, skin, pharyngeal tonsils, tracheobronchial lymph node, pre-scapular lymph node, mesenteric lymph node

10

M

Systemic congestion, severe; Necrosuppurative hepatitis, moderate; Lymphoplasmacytic encephalitis, mild; Granulomatous and eosinophilic lymphadenitis, bronchopneumonia, colitis and gastritis, moderate; Polysaccharide myocyte inclusions

Cerebrum, cerebellum (G, MN698991), spinal cord (G, MN698992), lung (MT770744), liver, spleen, kidney, skin (MT770743), pharyngeal tonsils (MT770742), tracheobronchial lymph node (MT770741), pre-scapular lymph node, mesenteric lymph node, penis (G, MN698993)

11

M

Systemic congestion, severe; Lymphoplasmacytic encephalitis, mild; lymphoplasmacytic hepatitis, mild; Granulomatous and eosinophilic lymphadenitis, bronchopneumonia, colitis, mild; Myonecrosis, mild

Cerebrum, cerebellum, spinal cord (A, MN699007), lung, liver, spleen, kidney, skin, pharyngeal tonsils (MT770748), tracheobronchial lymph node, pre-scapular lymph node, mesenteric lymph node, penis (G, MN698994)

12

M

Systemic congestion, severe; Lymphoplasmacytic encephalitis, mild; lymphoplasmacytic hepatitis, mild; Granulomatous and eosinophilic lymphadenitis, bronchopneumonia

Cerebrum, cerebellum, spinal cord, lung, liver, spleen, kidney, skin (MT770749), pharyngeal tonsils (MT770751), tracheobronchial lymph node (MT770750), pre-scapular lymph node, mesenteric lymph node, penis

13

M

Necrosuppurative hepatitis, moderate; Lymphoplasmacytic encephalitis, mild; Granulomatous and eosinophilic lymphadenitis as well as systemic congestion, severe

Cerebrum (G, MN698995), cerebellum (G, MN698996), spinal cord (G, MN698997), lung, liver, spleen, kidney, skin, pharyngeal tonsils (MT770746), tracheobronchial lymph node, pre-scapular lymph node, mesenteric lymph node (MT770747), penis (G, MN698998)

14

M

Systemic congestion, severe; Lymphoplasmacytic and histiocytic bronchopneumonia, moderate; Lymphoplasmacytic encephalitis, mild; Granulomatous and eosinophilic lymphadenitis, gastritis and duodenitis with intralesional trematodes, moderate

Cerebrum, cerebellum, spinal cord, lung, liver, spleen, kidney, skin (MT770740), pharyngeal tonsils, tracheobronchial lymph node, pre-scapular lymph node, mesenteric lymph node, penis (G, MN698999)

15

F

Systemic congestion, severe; Acute myonecrosis, severe; Granulomatous and eosinophilic lymphadenitis, pneumonia and colitis, mild; Lymphoplasmacytic encephalitis, mild; Lymphoplasmacytic cholangiohepatitis, mild

Cerebrum, cerebellum, spinal cord, lung, liver, spleen, kidney, skin (MT770745), pharyngeal tonsils, tracheobronchial lymph node, pre-scapular lymph node, mesenteric lymph node

16

Fa

Systemic congestion, severe; Pulmonary haemorrhage and atelectasis, severe; Granulomatous and eosinophilic lymphadenitis and gastritis

Cerebrum, cerebellum, spinal cord, lung, liver, spleen, kidney, skin, pharyngeal tonsils, tracheobronchial lymph node, pre-scapular lymph node, mesenteric lymph node

17

M

Systemic congestion, severe; Granulomatous and eosinophilic lymphadenitis, gastritis, colitis; Penile papilloma; Lymphoplasmacytic balanitis, mild

Cerebrum (G, MN699000), cerebellum (G, MN699001), spinal cord (G, MN699002), lung, liver, spleen, kidney, skin, pharyngeal tonsils, tracheobronchial lymph node, pre-scapular lymph node, mesenteric lymph node, penis (G, MN699003)

18

F

Systemic congestion, severe; Bacterial sepsis; Necrosuppurative hepatitis, moderate; Suppurative and haemorrhagic pneumonia, moderate; Acute myonecrosis, mild; Granulomatous and eosinophilic lymphadenitis and gastritis, mild

Cerebrum, cerebellum, spinal cord, lung, liver, spleen, kidney, skin, pharyngeal tonsils, tracheobronchial lymph node, pre-scapular lymph node, mesenteric lymph node,

19

M

Systemic congestion, severe; Lymphoplasmacytic and histiocytic meningitis, mild; Granulomatous and eosinophilic lymphadentitis and colitis

Cerebrum (G, MN699004), cerebellum, spinal cord (G, MN699005), lung, liver, spleen, kidney, skin, pharyngeal tonsils, tracheobronchial lymph node, pre-scapular lymph node, mesenteric lymph node, penis (G, MN699006)

  1. Samples positive by nested PCR are underlined. Accession numbers of herpesvirus DNA sequences are indicated in parentheses
  2. Abbreviations: A Alphaherpesvirus, F female, Fapregnant female, G Gammaherpesvirus, M male