Table 1 Clinical signs, analytical findings, cytology, serology, and rtPCR results recorded in the case of the two Eurasian otters

Dates of screening

August 2019 (diagnosis)

November 2019

(treatment)

November 2019

Clinical signs

Bilateral epistaxis, anorexia, apathy, weight loss, bilateral nephropathy with hydronephrosis, mesenteric lymphadenomegaly, ascites

Light hydronephrosis

No signs

 White blood cells (3.1–19.2 × 10³/mm³)

4.5

3

4.6

 Platelets (178–777 × 10³/mm³)

156

296

nd

 Total proteins (6–7.7 g/dL)^a

10.4

12.2

6.7

 Albumin (1.25–3.6 g/dL)^a

2.4

2.1

3.6

 Globulin (2.7–4.8 g/dL)^a

8

10

3.1

 PON-1 (3–4.3 IU/mL)^b

2.1

2.5

6.5

 Haptoglobin (<  3 g/L)^b

3.3

1.4

0.1

 CRP (<  12 μg/mL)^b

3.7

4

6.7

 Ferritin (60–190 μg/L)^b

2820

266

111

 Blood urea nitrogen (17.3–68.1 mg/dL)^a

37.1

nd

nd

 Serum creatinine (0.7–1 mg/dL)^a

0.7

nd

nd

 Urine protein/creatinine (UPC) ratio (<  0.2)

3

nd

nd

 Urine osmolarity (> 500 mOsm/L)

363

nd

nd

 Urine specific gravity (1.015–1.045)

1.012

nd

nd

Serology results by TR-IFMA (<  22 UFL)

266.6

229.4

4.84

Serology results by ELISA (<  1.1 S/P ratio)

4.9

nd

0.3

Spleen cytology (microscopy)

Presence of Leishmania spp. amastigotes

Presence of Leishmania spp. amastigotes

nd

 Peripheral blood

13.89

nd

NAC

 Bone marrow

222.81

nd

nd