Fig. 4
From: The first report of multidrug resistance in gastrointestinal nematodes in goat population in Poland
Polymorphism of the length of restriction fragments of variant-1 of the β-tubulin gene indicating the resistance of Haemonchus contortus to benzimidazoles (cropped gel). M – molecular-weight size marker pUC Mix (11–1444) (A&A Biotechnology, Gdańsk, Poland), L2, L4 – absence of gene fragment (250 bp) showing resistance for benzimidazoles (H. contortus), L1, L3, L5 – presence of gene fragment showing resistance for benzimidazoles (H. contortus), (1) – the first mixture with three single primers of a given species in ASA-PCR (Ph1 – forward primer, Ph4 – susceptible allele primer, Ph2 – reverse primer), (2) – the second mixture with three single primers of a given species in ASA-PCR (Ph1 – forward primer, Ph3 – resistant allele primer, Ph2 – reverse primer)