Cul o 2 specific IgG3/5 antibodies predicted Culicoides hypersensitivity in a group imported Icelandic horses

BMC Veterinary Research

Table 2 Culicoides allergens used for bead coupling to provide the allergen specific matrix of the allergen multiplex assay

*Culicoides* Species	Allergen	Fluorescent bead No.	Origin	Expression system
C. obsoletus	Cul o 2	40	Germany	Pichia pastoris
	Cul o 3 ^a	39	Germany	Pichia pastoris
	Cul o 3 (degly) ^a	37	Germany	Pichia pastoris
	Apyrase	41	Germany	Insect cells (sf+)
C. nubeculosus	Cul n 3	36	Iceland	Barley
	Cul n 4	34	Iceland	Barley
	Cul n 8	42	Germany	Pichia pastoris
C. sonorensis	Cul s 1	33	Cornell	E. coli
C. sonorensis	Cul s D7	38	Cornell	E. coli

^a Cul o 3 was used as originally expressed in Pichia pastoris. The same protein was also used in a deglycosylated form

ISSN: 1746-6148