Table 1 Primers used for qPCR
Genes Source/ accession No. | Accession number | Primer sequence (5′-3′) | Annealing temperature (°C) | Products (bp) |
|---|---|---|---|---|
RIG-I | NM_213804.2 | For: TCTGGAGCAACGTCTGACAC | 56 | 166 |
Rev: GTTTGCTGGTGTTGTGGCAT | ||||
MDA-5 | NM_001100194.1 | For: TGGACACAGCAGTGAGTTCAAGC | 51 | 105 |
Rev: GCCACCGTGGTAGCGATAAGC | ||||
MAVS | XM_005672763.1 | For: GGCATCAGAAGCAGGACACAGAAC | 59 | 177 |
Rev: CAGTGGAGGAGGAGGCAGTAGAC | ||||
IκBα | NM_001005150.1 | For: CCAACCAGCCAGAAATCGCT | 58 | 168 |
Rev: GCAGAATGGAGTGGAGGTGC | ||||
c-Jun | NM_213880.1 | For: GCATCGCTGCCTCCAAGT | 56 | 224 |
Rev: CCCAACAGTCTCGCCTCAAA | ||||
IL-8 | NM_213867 | For: TCCTGCTTTCTGCAGCTCTC | 59 | 100 |
Rev: GGGTGGAAAGGTGTGGAATG | ||||
β-actin | XM_003357928 | For: TCATCACCATCGGCAACT | 58 | 133 |
Rev: TTCCTGATGTCCACGTCGC |