Age related differences in phylogenetic diversity, prevalence of Shiga toxins, Intimin, Hemolysin genes and select serogroups of Escherichia. coli from pastured meat goats detected in a longitudinal cohort study

Table 3 Primers used in the study

Fragment size (bp)	Target gene	Sequence (5′-3′)	Primer	Ref
338	Stx1	TCTCAGTGGGCGTTCTTATG	Stx1-a	[57]
338	Stx1	TACCCCCTCAACTGCTAATA	Stx1-b	[57]
115	Stx2(EHEC)	GCGGTTTTATTTGCATTAGC	Stx2-a	[57]
115	Stx2(EHEC)	TCCCGTCAACCTTCACTGTA	Stx2-b	[57]
248	eaeA	ATGCTTAGTGCTGGTTTAGG	EAEA-a	[57]
248	eaeA	GCCTTCATCATTTCGCTTTC	EAEA-b	[57]
569	hlyA	AGCTGCAAGTGCGGGTCTG	HlyA-a	[57]
569	hlyA	TACGGGTTATGCCTGCAAGTTCAC	HlyA-b	[57]
152	O26wzx	GCGCTGCAATTGCTTATGTA	Wzx-F	[72]
152	O26wzx	TTTCCCCGCAATTTATTCAG	Wzx-R	[72]
527	O45wzx	CCGGGTTTCGATTTGTGAAGGTTG	Wzx-F	[72]
527	O45wzx	CACAACAGCCACTACTAGGCAGAA	Wzx-R	[72]
321	O103wzx	TTGGAGCGTTAACTGGACCT	Wzx-F	[72]
321	O103wzx	GCTCCCGAGCACGTATAAG	Wzx-R	[72]
925	O126wzx	TTAGCTCTCGTAGAGGCTGGTGTT	Wzx-F	[72]
925	O126wzx	ATGTCATTCCTGGGACGCGAATGT	Wzx-R	[72]
640	O146wzx	AGGGTGACCATCAACACACTTGGA	wzx-F	[72]
640	O146wzx	AGTTCAATACTGTCGCAGCTCCTC	wzx-R	[72]
118	Stx2	GTGCCTGTTACTGGGTTTTTCTTC	Stx2F	[53]
118	Stx2	AGGGGTCGATATCTCTGTCC	Stx2R	[53]
211	yjaA	TGAAGTGTCAGGAGACGCTG	YjaA.1	[32]
211	yjaA	ATGGAGAATGCGTTCCTCAAC	YjaA.2	[32]
152	tspE4.C2	GAGTAATGTCGGGGCATTCA	TspE4C2.1	[32]
152	tspE4.C2	CGCGCCAACAAAGTATTACG	TspE4C2.2	[32]
279	chuA	GACGAACCA ACGGTCAGGAT	ChuA.1	[32]
279	chuA	TGCCGCCAGTACC AAAGACA	ChuA.2	[32]
401	E. coli 16 s	CCCCCTGGACGAAGACTGAC	EC16S-a	[57]
401	E. coli 16 s	ACCGCTGGCAACAAAGGATA	EC16S-b	[57]

ISSN: 1746-6148