| |
CONTROL
|
ISCHEMIA WITHOUT REPERFUSION
|
1-WEEK REPERFUSION
|
1-MONTH REPERFUSION
|
|---|
| |
NECROTIC
|
SALVAGED
|
NECROTIC
|
SALVAGED
|
NECROTIC
|
SALVAGED
|
|---|
|
Collagen I (%)
|
20.3 ± 10.1
|
24.3 ± 6.7
|
18.9 ± 12.1
|
53.0 ± 10.1**
|
18.1 ± 12.0
|
67.1 ± 3.8**
|
23.0 ± 3.1
|
|
Collagen III (%)
|
16.0 ± 9.3
|
21.9 ± 8.5
|
21.6 ± 8.4
|
24.6 ± 8.5
|
14.5 ± 8.5
|
36.6 ± 14.6*
|
17.9 ± 7.7
|
|
Elastic fibers (%)
|
6.7 ± 1.9
|
11.2 ± 3.1*
|
9.1 ± 1.2*
|
20.3 ± 6.2**
|
13.6 ± 7.5
|
16.4 ± 3.0**
|
12.6 ± 7.4
|
|
GAG (%)
|
10.9 ± 7.1
|
5.9 ± 1.4
|
5.1 ± 1.3
|
29.2 ± 5.0**
|
16.4 ± 10.0
|
25.4 ± 8.3**
|
14.1 ± 6.3
|
|
SPARC (%)
|
2.5 ± 0.6
|
0.4 ± 0.4
|
2.1 ± 0.5
|
6.9 ± 2.0**
|
1.4 ± 0.9
|
6.6 ± 1.2**
|
1.2 ± 0.3
|
|
Perlecan (%)
|
0.7 ± 0.4
|
0.7 ± 0.2
|
0.9 ± 0.2
|
0.3 ± 0.2
|
0.1 ± 0.2
|
1.1 ± 0.7
|
0.4 ± 0.4
|
|
Laminin (%)
|
8.7 ± 0.9
|
19.8 ± 1.4**
|
19.5 ± 6.2**
|
14.3 ± 1.5**
|
11.5 ± 1.3*
|
17.7 ± 1.1**
|
5.5 ± 4.2
|
|
Fibronectin (%)
|
0.1 ± 0.1
|
2.0 ± 0.8**
|
0.5 ± 0.4*
|
13.3 ± 9.8*
|
0.6 ± 0.7
|
7.2 ± 4.1**
|
0.4 ± 0.6
|
- Data were expressed as mean ± SD (n ≥ 5) and were analysed by unpaired t-Student’s test. *P < 0.05, **P < 0.01 vs. control
- Abbreviations: ECM Extracellular matrix; GAG Glycosaminoglycan; SPARC Secreted protein acidic and rich in cysteine
