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Table 2 Antibodies used in immunohistochemical analysis

From: Immunohistochemical detection of p53 and pp53 Ser392 in canine hemangiomas and hemangiosarcomas located in the skin

Antigen Clone Source Dilution Type of Antibody Detection System/Source
CD31 JC/70A DAKO, Copenhagen, Denmark 1:100 Mouse MAb Vectastain Elite, ABC Kit; Vector Laboratories, Burlingame, California, USA
Ki67 MIB-1 DAKO, Copenhagen, Denmark 1:100 Mouse MAb Vectastain Elite, ABC Kit; Vector Laboratories, Burlingame, California, USA
p53 CM1 Signet Laboratories, Dedham, MA 1:200 Rabbit PAb EnVision+ System Labelled Polymer-HRP anti-rabbit (Dako, Copenhagen, Denmark)
pp53 Ser392 sc-56,173 Santa Cruz Biotechnology Inc., Santa Cruz, CA 1:100 Mouse MAb Vectastain Elite, ABC Kit; Vector Laboratories, Burlingame, California, USA
  1. MAb monoclonal antibody, PAb polyclonal antibody
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