The addition of Lactobacillus spp. negatively affects Mycoplasma bovis viability in bovine cervical mucus

BMC Veterinary Research

Table 3 Least squares means of pH and log CFU/mL of M. bovis and LAB by time

Condition	Composition	Hour (h)	pH¹	Log CFU/ml of M. bovis²	Log CFU/ml of LAB³
1	CM + MB	0	8.40^a	7.23^a	0^a
		15	7.52^a	7.16^a	2.22^a
2	CM + L1	0	8.40^a	–	1.81^a
		15	6.64^b	–	7.40^b
3	CM + L2	0	8.40^a	–	3.84^a
		15	5.60^b	–	11.66^b
4	CM + MB + L1	0	8.40^a	6.44^a	2.41^a
		15	6.73^b	7.65^b	7.32^a
5	CM+ MB + L2	0	8.40^a	7.56^a	3.20^a
		15	5.60^b	0^b	9.75^b
6	SP4 + MB + L1	0	7.60^a	7.38^a	1.93^a
		15	7.19^a	8.72^b	4.34^a
7	SP4 + MB + L2	0	7.60^a	7.45^a	3.31^a
		15	7.21^a	8.19^b	5.44^a

^{a, b}: Means with different superscripts between the times within conditions differ significantly (P < 0.05).¹Standard error of the mean (SEM): 0.34; ²SEM: 0.11; ³ SEM: 1.86. CM: cervical mucus. MB: M. bovis. SP4: Specific medium for Mycoplasma spp. isolation (conditions without CM). L1: Lactobacillus spp. less concentrated (3.24 × 10⁶ UFC/mL). L2: Lactobacillus spp. more concentrated (3.24 × 10⁸ UFC/mL). LAB: Lactic acid bacteria