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Table 1 Experimental conditions evaluated

From: The addition of Lactobacillus spp. negatively affects Mycoplasma bovis viability in bovine cervical mucus

Condition

Composition

M. bovis

LAB

1

CM (1460 μL) + MB (40 μL)

☑*

2

CM (1460 μL) + L1 (40 μL)

 

3

CM (1000 μL) + L2 (500 μL)

 

4

CM (1420 μL) + MB (40 μL) + L1 (40 μL)

5

CM (960 μL) + MB (40 μL) + L2 (500 μL)

6

SP4 (1420 μL) + MB (40 μL) + L1 (40 μL)

7

SP4 (960 μL) + MB (40 μL) + L2 (500 μL)

  1. CM: Cervical mucus. MB: M. bovis. SP4: Specific medium for Mycoplasma spp. isolation (conditions without CM). L1: Lactobacillus spp. at a concentration of 3.24 × 106 UFC/mL. L2: Lactobacillus spp. at a concentration of 3.24 × 108 UFC/mL. LAB: Lactic acid bacteria. ☑: Counts of M. bovis and lactic acid bacteria were made.* Lactic acid bacteria were isolated in the cervical mucus pool. The experimental conditions were prepared following a protocol previously described [25]