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Table 3 aELISA results, sensitivities and specificities of antigens on sera from naive, vaccinated and CBPP-infected cattle

From: Baseline analysis of Mycoplasma mycoides subsp. mycoides antigens as targets for a DIVA assay for use with a subunit vaccine for contagious bovine pleuropneumonia

  Test (Antigen) Controlb Sera (21 days) after boost Se (%) Sp (%) PPV (%) NPV (%)
   +Ve -Ve +Ve -Ve     
Vaccine MSC_0136 0/8 8/8 7/8 1/8 87.5 100 100 88.9
MSC_0431 0/8 8/8 8/8 0/8 100 100 100 100
MSC_0499 0/8 8/8 8/8 0/8 100 100 100 100
MSC_0775 0/8 8/8 7/8 1/8 87.5 100 100 88.9
MSC_0776 0/8 8/8 5/8 3/8 62.5 100 100 72.7
MSC_0957 0/8 8/8 0/8 8/8 0 100 0 50
    Sera (8–20 weeks) after infection     
Non-vaccine MSC_0397 0/8 8/8 5/8 3/8 62.5 100 100 72.7
MSC_0636 0/8 8/8 7/8 1/8 87.5 100 100 88.9
MSC_0653 0/8 8/8 7/8 1/8 87.5 100 100 88.9
LppB 0/8 8/8 7/8 1/8 87.5 100 100 88.9
  1. aThis table represents results through the use of sera from naïve, CBPP-infected, and subunit-vaccinated cattle only. +Ve., positive; −Ve., negative. Cohen’s kappa index value, classified as very good (1–0.76), good (0.75–0.61), acceptable (0.6–0.4), and poor (<0.4)
  2. bAll CBPP-negative serum samples obtained from a group of 8 cattle (n = 8) were correctly identified as negative