Skip to main content

Table 2 Primer and probe sequences used for qRT-PCR APPV detection, in accordance with previously published protocols, primers and probes [6, 12]. The primer and probe, denoted ā€œSweā€ are slightly modified in accordance with [13]

From: Detection of atypical porcine pestivirus in Swedish piglets with congenital tremor type A-II

Oligo name

Sequence (5ā€²-3ā€²)

APPV-NS5B-303F

GTAGGGCGGATACAGAAATA

APPV-NS5B-385R

GGYACTTCCTCCATCATGG

APPV_5587-fw (NS3)

CAGAGRAAAGGKCGAGTGGG

APPV_5703_Swe-rev (NS3)

ACCATACTCTTGRGCCTGCAG

APPV_5087-fw (NS3)

GAAAGTGTCTGCCGCTTCATG

APPV-NS5B-336-FAM

AAATATTGGAAATYYATTGACAATTTGAC

APPV_Swe probe

ACTACTATCCTTCGGGGGTRGTRCCGA