Development of a computer-based quantification method for immunohistochemically-stained tissues and its application to study mast cells in equine wound healing (proof of concept)

Table 1 Validation of the quantification method using ICCs (n = 6)

Comparisons	ICC	p
Same operator	0.90	0.00
Two different operators	0.69	0.01
Mean of ImageJ vs pathologist	0.67	0.04

Pathologist: manual counting; ICC Intraclass correlation coefficient; The ICC calculated between the ImageJ method and the pathologist-based manual counting method used the mean of the 2 counts done by each method p < 0.05 was considered statistically significant

ISSN: 1746-6148