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Table 1 Validation of the quantification method using ICCs (n = 6)

From: Development of a computer-based quantification method for immunohistochemically-stained tissues and its application to study mast cells in equine wound healing (proof of concept)

Comparisons ICC p
Same operator 0.90 0.00
Two different operators 0.69 0.01
Mean of ImageJ vs pathologist 0.67 0.04
  1. Pathologist: manual counting; ICC Intraclass correlation coefficient; The ICC calculated between the ImageJ method and the pathologist-based manual counting method used the mean of the 2 counts done by each method p < 0.05 was considered statistically significant