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Fig. 3 | BMC Veterinary Research

Fig. 3

From: Development of a computer-based quantification method for immunohistochemically-stained tissues and its application to study mast cells in equine wound healing (proof of concept)

Fig. 3

Photographs and quantification of CD117+ mast cells in skin wounds of horses. a Photographs showing a a) HPF of an equine CD117+ skin mast cell (400x) and b) mast cells distribution in the i-subepidermal and ii-deep dermal layer of the intact skin borders and in the c) granulation tissue of a 3-day old thoracic wound. Immunohistochemistry was performed with the avidin-biotin-alkaline phosphatase method and used Harris hematoxylin counter stain (× 200). Arrow: epidermis, arrowhead: blood vessel, asterisk: sweat gland. b Graphs showing evolution of mast cells numbers (/mm2) over time and according to anatomical location in the subepidermal layer, deep dermal layer, and granulation tissue (left to right). Values represent mean ± standard error of the mean (SEM) (n = 26)

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BMC Veterinary Research

ISSN: 1746-6148

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