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Fig. 2 | BMC Veterinary Research

Fig. 2

From: Development of a computer-based quantification method for immunohistochemically-stained tissues and its application to study mast cells in equine wound healing (proof of concept)

Fig. 2

Comparison of the variation between the ImageJ and the pathologist manual counting method. a Bland-Altman plot of the difference in cell counts between the ImageJ method and the pathologist manual counting method compared to the average (n = 6). The difference in cell counts corresponds to the difference between the mean of two counts done by each method. b Data represent the coefficient of variation expressed in percentage between two counts. White circles represent the pathologist manual-based counting method and black circles represent the ImageJ computer-based method. Statistical analysis was done on paired data for each specimen (n = 6). Lines and bars represent the mean ± standard deviation (SD). Upper and lower dashed lines correspond to the upper and lower 95% agreement limits. %CV: coefficient of variation in percentage

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