Fig. 5

nMDS plot based on Bray-Curtis distance matrix of ruminal microbiome functions illustrates the effect of subacute ruminal acidosis (SARA) challenge and Saccharomyces cerevisiae fermentation products (SCFP) supplementation from shotgun metagenomic sequencing. The effects of SARA challenge, SCFP supplementation and their interaction were significant (P < 0.05)

. Abbreviations in figure: N_C, normal diet without SCFP supplementation; N_S, normal diet with SCFP supplementation; S_C, SARA challenge without SCFP supplementation; S_S, SARA challenge with SCFP supplementation.