Fig. 4

nMDS plot based on Bray-Curtis distance matrix illustrates variation in rumen bacterial communities affected by subacute ruminal acidosis (SARA) challenge and supplementation of Saccharomyces cerevisiae fermentation products (SCFP) from shotgun metagenomic sequencing. The significance of the effects of SARA challenge, SCFP supplementation and their interaction were P < 0.05, P < 0.05, and P < 0.42, respectively. Abbreviations in the figure: N_C, normal diet without SCFP supplementation; N_S, normal diet with SCFP supplementation; S_C, SARA challenge without SCFP supplementation; S_S, SARA challenge with SCFP supplementation