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Table 1 The primer and probe sequences

From: Optimized real-time fluorescence PCR assay for the detection of porcine Circovirus type 3 (PCV3)

Methods Primer and probe sequences   Length Readout
PCR F:5′-CCA CAG AAG GCG CTA TGT C-3′
R: 5′-CCG CAT AAG GGT CGT CTT G-3′
Palinski, 2016 [1] 330 bp Electrophoresis
qPCR F: 5′-CGCATAAGGGTCGTCTTGGA-3′
R: 5′-CMGCTCAGCAAACAAAAACTATGTTC-3′
P: 5′-FAM-TCCAGGCGCCGTCTAGATCTATGGC-BHQ1–3′
This study 95 bp Real-time fluorescence
qPCR F: 5′-CGGACTTGTAACGAATCCAAACT-3′
R: 5′-GGAGCATTTATGCCCCGGAAA-3′
P: FAM-5′-CTTTSGTGCCGTAGAAGTCTGTCATTCCA-3′-Eclipse
Wang, 2017 [7] 78 bp Real-time fluorescence
  1. F forward primer, R reserve primer, P probe