From: Optimized real-time fluorescence PCR assay for the detection of porcine Circovirus type 3 (PCV3)
Methods | Primer and probe sequences | Length | Readout | |
---|---|---|---|---|
PCR | F:5′-CCA CAG AAG GCG CTA TGT C-3′ R: 5′-CCG CAT AAG GGT CGT CTT G-3′ | Palinski, 2016 [1] | 330 bp | Electrophoresis |
qPCR | F: 5′-CGCATAAGGGTCGTCTTGGA-3′ R: 5′-CMGCTCAGCAAACAAAAACTATGTTC-3′ P: 5′-FAM-TCCAGGCGCCGTCTAGATCTATGGC-BHQ1–3′ | This study | 95 bp | Real-time fluorescence |
qPCR | F: 5′-CGGACTTGTAACGAATCCAAACT-3′ R: 5′-GGAGCATTTATGCCCCGGAAA-3′ P: FAM-5′-CTTTSGTGCCGTAGAAGTCTGTCATTCCA-3′-Eclipse | Wang, 2017 [7] | 78 bp | Real-time fluorescence |