Fig. 4
From: Gene expression of prostaglandin EP4 receptor in three canine carcinomas
Analysis of ptger4 transcription in a section of normal bladder (a-c) and transitional cell carcinoma (d-f) following RNAscope® mRNA in situ hybridization with hematoxylin counter stain. a & d: Native photomicrograph to be analyzed for copy number/cell, H-Score, and percentage transcript expression using HALO software with RNAscope® Modules. b & e: Target cells were manually gated (yellow line) for analysis. c & f: HALO generated probe markup from which copy number/cell, H-Score, and percentage transcript expression are calculated