Rapid differentiation of PEDV wild-type strains and classical attenuated vaccine strains by fluorescent probe-based reverse transcription recombinase polymerase amplification assay

Table 3 Sequence of primers and probes for PEDV real-time RT-RPA method

Name	Sequence(5′-3′)	Location	Product size (bp)
F27313	TCGTGAGCTAGCGGACTCTTACGAGATTAC	N,27313-27342^a
R27453	GCTGCAGCGTGGTTTCACGCTTGTTCTTCT	N,27453-27482^a	170 bp
P27375	ATCCAAATGTTGAGCTTCTTGTTTCACAGG (FAM-dT) G (THF) A (BHQ1-dT)GCATTTAAAACTGGG-C3 space	N,27375-27424^a
F3330	GTGATGAAGTAGACTCCTCTGACCCTGATA	ORF1,3330-3359^a
R3467	CTTAGTAACTGTGGAAGGTGTATCTTTAAT	ORF1,3467-3496^a	167 bp
P3364	GGCAGATGTGGCTAACTCTGAAACTGAGGC (FAM-dT) GA (THF) GA (BHQ1-dT)GTAGAGTCTGAAGTT-C3 space	ORF1,3364-3413^a
F_ORF1	CACCGATCCTAATCTGCCCG	ORF1,3217-3236^a
R_0RF1	TGGACCAACTCTACCAGCAC	ORF1,3612-3632^a	415 bp

a. PEDV calssical attenuated vaccine CV777 strain, GenBank accession number KT323979

ISSN: 1746-6148