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Fig. 5 | BMC Veterinary Research

Fig. 5

From: Study of the immunogenicity of the VP2 protein of canine parvovirus produced using an improved Baculovirus expression system

Fig. 5

Purification and characterization of recombinant VP2 protein. a The SDS-PAGE analysis of purified recombinant VP2 protein. Lane M: PageRuler™ Prestained Protein Ladder, 10 to 180 kDa; Lane 1: ultrasound supernatant of the recombinant baculovirus Ac-IM-ph-VP2 + p10-VP2 infected Sf9 cells; Lane 2: 200 mM Imidazole eluent; Lane 3: 300 mM Imidazole eluent. b Western blot analysis of purified VP2 protein with Mouse anti-His monoclonal antibodies. Lane 1: purified VP2 protein; c Western blot analysis of purified VP2 protein with Rabbit anti-VP2 polyclonal antibodies. Lane 1: purified VP2 protein

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BMC Veterinary Research

ISSN: 1746-6148

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