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Fig. 3 | BMC Veterinary Research

Fig. 3

From: An insight into the transmission role of insect vectors based on the examination of gene characteristics of African swine fever virus originated from non-blood sucking flies in pig farm environments

Fig. 3

Protein interaction and gene expression analysis. An interactome map was constructed for the Bcl-2 protein, a homolog of A179L. Potential interaction partners of Bcl-2 with a high confidence score (C0.700) were predicted by STRING tool and verified by transfection and fluorescence qPCR. a Predicted map of interaction partners between Bcl-2 protein and A179L homolog from swine/pig. b Western blot analysis (cropping gels) of protein expression at different times after ASFV PEGX-A179L transfection into HEK-293 T cells. cBcl-2 mRNA transcription levels at different times after ASFV PEGX- A179L from M. domestica flies and Georgia/2007 being transfected into HEK-293 T cells, respectively. dBeclin1 mRNA transcription levels at different times after ASFV PEGX-A179L from M. domestica flies and Georgia/2007 being transfected into HEK-293 T cells, respectively

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