Fig. 7From: Inhibiting autophagy potentiates the antitumor efficacy of Euphorbia royleana for canine mammary gland tumorsBlocking autophagy enhanced X.E.E. and X.H.E cell death. (a) Pre-culture cells with different concentration of autophagy inhibitor, Baf A1, then culture MPG and CMT1 cells with X.E.E. 10 μg/ml or X.H.E. 3 μg/ml for 2 days. Cell viability was determined after 2 days by WST1 assay. (b) The cells were treated with bafilomycin (Baf) (6 nM) for 4 h and microtubule-associated protein 1 light chain 3 (LC3-I & II) expression was measured to demonstrate the autophagy-blocking ability of Baf A1Back to article page