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Table 2 Intra- and Inter- assay reproducibility of the triplex real-time PCR

From: Development and application of a triplex real-time PCR assay for simultaneous detection of avian influenza virus, Newcastle disease virus, and duck Tembusu virus

Name

Number of DNA Copies (copies/μL)

Intra-assay

Inter-assay

Mean

SD

CV (%)

Mean

SD

CV (%)

AIV

1 × 107

13.71

0.27

1.97

13.52

0.60

4.44

1 × 105

20.54

0.22

1.07

20.22

0.25

1.24

1 × 103

25.97

0.35

1.35

26.14

0.61

2.33

NDV

1 × 107

14.72

0.07

0.48

14.64

0.27

1.84

1 × 105

21.44

0.31

1.45

21.50

0.34

1.58

1 × 103

28.11

0.35

1.25

27.90

0.76

2.72

DTMUV

1 × 107

14.25

0.12

0.84

14.38

0.33

2.29

1 × 105

21.35

0.35

1.64

21.46

0.36

1.68

1 × 103

28.25

0.62

2.19

28.33

0.89

3.14