Fig. 5

Immunohistochemical localization of PDPN in HSA and normal splenic samples. a PDPN detection in normal splenic tissue was low to absent (B297, normal white pulp). b anti-PDPN staining appears weak in endothelial cells of blood vessels that are adjacent to tumor masses (B783). c and d anti-PDPN staining is strong in cells within the tumor’s vascular regions (B848). In these regions the PDPN-positive tumor cells were seen to surround what appears to be collagen bundles. PDPN was also detected in tumor cells from the more avascular regions of the tumor (Supplemental materials). c = low magnification, d = high magnification. Scale bar represents 50 μm