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Fig. 1 | BMC Veterinary Research

Fig. 1

From: The influence of dietary supplementation with the leucine metabolite β-hydroxy-β-methylbutyrate (HMB) on the chemotaxis, phagocytosis and respiratory burst of peripheral blood granulocytes and monocytes in calves

Fig. 1

a. The chemotactic index was calculated by dividing the number of cells that migrated towards fMLP by the number of cells that migrated in the absence of fMLP. Key: I – control group; II – experimental group; SD - standard deviation; Numerical results were presented as the arithmetic mean ± SD. The significance level was set at 0.05. Asterisks refer to statistically significant differences between the control group and the experimental group on the same sampling day at ** p < 0.01; *** p < 0.001; C refers to statistically significant differences between day “0” and the consecutive sampling days in the experimental group at C – p < 0.001. b. Representative dot plot cytograms showing the percentage of migrating granulocytes and counting beads on day 30 of the experiment. (a) – control without stimulation; (b) – control stimulated with fMLP; (c) – HMB without stimulation; (d) – HMB stimulated with fMLP. The number of granulocytes relative to the number of whole cells was determined in each sample as soon as 2000 counting beads were acquired. Stimulation with fMLP increased the number of migrated granulocytes (b), (d) relative to the controls without fMLP (a), (c)

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BMC Veterinary Research

ISSN: 1746-6148

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