Table 3 Sequences of the primers and probes for M .ovipneumoniae real-time RPA, LFS RPA and PCR assays
Assay | Primers and probes | Sequence 5′-3′ | Amplicon size (bp) | References |
|---|---|---|---|---|
real-time RPA | MO-exo-F | TGAGTAACACGTACCTAACCTACCTTTTGGAC | 254 | This study |
MO-exo-R | TGCTGCCTCCCGTAGGAGTCTGGGCCGTATCTC | |||
MO-exo-P | TTGGTAGGGTAAAGGCCTACCAAGACGATGA (FAM-dT)(THF)(BHQ1-dT)TTAGCGGGGCCAAGAG-C3-spacer | |||
LFS RPA | MO-nfo-F | TGAGTAACACGTACCTAACCTACCTTTTGGAC | 254 | This study |
MO-nfo-R | Biotin-TGCTGCCTCCCGTAGGAGTCTGGGCCGTATCTC | |||
MO-nfo-P | FAM-TTGGTAGGGTAAAGGCCTACCAAGACGATGAT(THF)TTTAGCGGGGCCAAGAG-C3-spacer | |||
real-time PCR | Mo16S_35F | TGGGTGAGTAACACGTACCTAACC | 62 | [4] |
Mo16S_96R | AGCCGCTGTTTCCAATGG | |||
Mo16S_60T | FAM-ACCTTTTGGACCGGGATA-MGB | |||
PCR | LMF1 | TGAACGGAATATGTTAGCTT | 361 | [9] |
LMR1 | GACTTCATCCTGCACTCTGT |