Fig. 3From: Development and validation of the isothermal recombinase polymerase amplification assays for rapid detection of Mycoplasma ovipneumoniae in sheepReproducibility of M. ovipneumoniae real-time RPA assay. The analytical sensitivity was determined on DNA molecular standard (8 runs) for real-time RPA. Semi-logarithmic regression of the data collected from real-time RPA test runs on the DNA molecular standards using Prism Software. The run time of the real-time RPA was between 4 min–13 min for 1.0 × 107–1.0 × 102 copies M. ovipneumoniae standard DNABack to article page