Differential expression of hemolysin genes in weakly and strongly hemolytic Brachyspira hyodysenteriae strains

BMC Veterinary Research

Table 2 Differences in hemolysin gene transcription activities between weakly hemolytic B. hyodysenteriae strain G423 and strongly hemolytic strain B204 determined with the software REST®

Hemolysin gene	Relative expression report of G423 (control strain) compared with B204 (sample strain) determined by the software REST®
	Time point 1			Time point 2			Time point 3			Time point 4
	Expression	p-value	Result	Expression	p-value	Result	Expression	p-value	Result	Expression	p-value	Result^a
tlyA	1.191	0.795		21.148	0.004	UP	1.403	0.549		0.919	0.881
tlyB	0.490	0.003	DOWN	1.493	0.413		0.168	0.000	DOWN	1.489	0.103
tlyC	1.698	0.380		6.815	0.040	UP	0.288	0.042	DOWN	0.894	0.807
hlyA	0.257	0.146		0.617	0.250		0.035	0.000	DOWN	0.573	0.143
hemolysin III	2.799	0.041	UP	6.269	0.004	UP	0.743	0.485		0.350	0.001	DOWN
hemolysin activa-tion protein gene	0.378	0.372		1.234	0.599		2.110	0.050	UP	2.054	0.011	UP
hemolysin channel protein gene	0.927	0.873		1.501	0.240		0.490	0.001	DOWN	1.542	0.010	UP
hemolysin gene	22.245	0.000	UP	6.088	0.004	UP	1.596	0.173		1.668	0.412

^aDOWN = hemolysin gene is downregulated in B204 in comparison with G423 by the mean factor described in the field “expression”, UP = hemolysin gene is upregulated in B204 in comparison with G423 by the mean factor described in the field “expression”

ISSN: 1746-6148