Pulmonary and systemic responses to aerosolized lysate of Staphylococcus aureus and Escherichia coli in calves

Table 1 Bronchoalveolar lavage fluid proteins that were significantly elevated following aerosolization of bacterial lysate relative to baseline

Accession	Description	Ratio of post-lysate to basline	P-value
G3MYZ3	Afamin	2.06	0.04
Q7SIH1	Alpha-2-macroglobulin	3.05	0.04
P01888	Beta-2-microglobulin	2.25	0.00
Q0V8R6	Beta-hexosaminidase subunit alpha	1.67	0.01
F1N076	Ceruloplasmin	2.92	0.04
P81187	Complement factor B	2.22	0.00
Q28085	Complement factor H	3.39	0.04
A5PJT7	ECM1 protein	2.78	0.02
Q29437	Primary amine oxidase, liver isozyme	3.23	0.01
P82943	Regakine-1	2.50	0.04
Q29443	Serotransferrin	1.92	0.01
G3N1U4	Serpin A3–3	2.46	0.05
Q2KIS7	Tetranectin	3.02	0.04
F1MMR5	Tetratricopeptide repeat domain 38	2.30	0.03
Q3ZBS7	Vitronectin	2.24	0.02
E1BH06	Uncharacterized protein^a	2.46	0.05
F1MCF8	Uncharacterized protein	1.40	0.02
F1MJK3	Uncharacterized protein	4.34	0.04
F1MVK1	Uncharacterized protein	4.19	0.05

The table shows bronchoalveolar lavage fluid proteins that were significantly elevated (n = 19) following aerosolization of bacterial lysate relative to baseline, as determined by a paired t-test. Tandem mass-tag mass spectrometry was performed on bronchoalveolar lavage fluid from 4 calves obtained at baseline and 24 h after aerosol administration of bacterial lysate, with data analysis and protein normalization in Proteome Discoverer 2.2 against Uniprot Bovine Database
^aFour uncharacterized proteins were increased following treatment

ISSN: 1746-6148