Fig. 1
Effect of bacterial lysate on relative tracheal antimicrobial peptide (TAP) and lingual antimicrobial peptide (LAP) gene expression in tracheal epithelial cells.Primary cultures of bovine tracheal epithelial cells were exposed for 16 h to medium treated with saline (negative control) or lysate of Staphylococcus aureus and Escherichia coli (107 CFU-equivalents of each). Following exposure, TAP, LAP and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) gene expression were measured using RT-qPCR. Data are presented as the mean normalized TAP: GAPDH or LAP:GAPDH ratios plus or minus the standard error of the mean. Treatment with bacterial lysate resulted in an average of 15-fold greater TAP expression (P = 0.002; Fig. 1a) and 13-fold greater LAP expression (P = 0.018; Fig. 1b) compared to saline-treated cells. The data represent tracheal epithelial cells from 5 calves (TAP) and 3 calves (LAP)