Multilocus sequence typing characterizes diversity of Ureaplasma diversum strains, and intra-species variability induces different immune response profiles

Table 1 Genes selected for MLST scheme and virulence analysis, with their respective primers designed from access to categorized genes of U. diversum ATCC 49782. F indicates forward and R indicates reverse

Gene	Primer	Primer Sequence 5′ – 3’	Gene length	Amplicon
MLST scheme
ftsH	ftsH - F	CAGTGCGTGATAACAAAACTG	1533 bp	753 bp
ftsH	ftsH - R	CAACTACATCAGCTTCTTCAGC	1533 bp	753 bp
polC	polC – F	ACGGATCTCAGGTTTATCTCAT	4305 bp	653 bp
polC	polC – R	CAGGAAGCATTGTAAAAGGTG	4305 bp	653 bp
rpL22	rpL22 – F	CTCAGCAGTTGCTAATGTAACT	972 bp	809 bp
rpL22	rpL22 – R	AGTATTGGAACCCACTTGG	972 bp	809 bp
rpoB	rpoB – F	AGCCGTATGAACATTGGAC	4281 bp	718 bp
rpoB	rpoB – R	ACTTGAATGATTCAGGCATTCC	4281 bp	718 bp
ureA	ureA – F	CGACCTTGCTCGTAGACGT	306 bp	248 bp
ureA	ureA – R	AGATAGGTTCGTGAATTGACAC	306 bp	248 bp
valS	valS – F	TGCTCCTTGTATTAGTGAAGAC	2616 bp	468 bp
valS	valS – R	GCTGGGGCTTTAGATGTAAA	2616 bp	468 bp
Virulence genes
pld	pld – F	CCAACTGAAGAGTTGATTGTAGC	615 bp	396 bp
pld	pld – R	GGTTTCAACAACCTGTCTTGCG	615 bp	396 bp
tgl	tgl – F	ATTGCTTGTATGCTTGCGGAAG	873 bp	546 bp
tgl	tgl – R	ATCACCCTCTGATTCTTCAACT	873 bp	546 bp
hlyA	hlyA – F	GGAGTTGTTCGTTATGTTGGA	1389 bp	463 bp
hlyA	hlyA – R	CAACAAGCCGACTAAATCCAGT	1389 bp	463 bp
mib	mib – F	GATACACCGATTGAACCTCCA	2484 bp	924 bp
mib	mib – R	TCAACCAGGATAACTTCCTTGA	2484 bp	924 bp
mip	mip – F	ACGAATTTGAAGGTAAGGTAGC	2400 bp	539 bp
mip	mip – R	TCAACTTGCTTGTTGACCAGG	2400 bp	539 bp
mba	mba – F	GTTGGTGCTATTATGGCAGGG	1398 bp	704 bp
mba	mba – R	CCATTGTTTGGACTAGGAGTT	1398 bp	704 bp
VsA	VsA – F	AACCTAATCTTGGTTCTGACC	1227 bp	255 bp
VsA	VsA – R	CTTCGCTTCCTGAACCTTCCAT	1227 bp	255 bp
tABC	tABC – F	CGCTTTATTTGCTGCGGTATTA	936 bp	683 bp
tABC	tABC – R	CAAGTGCTAAGAATCCAGCTCC	936 bp	683 bp

ISSN: 1746-6148