Molecular detection of porcine reproductive and respiratory syndrome virus, porcine circovirus 2 and hepatitis E virus in oral fluid compared to their detection in faeces and serum

Table 3 Specific primers and probes used in this study

Detected virus	Name	Primer and probe sequence (5′-3′)	PCR product	Reference
PRRSV	F-P1	CCA GCC AGT CAA TCA RCT GTG	291 bp	Donadeu et al., 1999 [36]
PRRSV	R-P2	GCG AAT CAG GCG CAC WGT ATG	291 bp	Donadeu et al., 1999 [36]
PCV2	2A	CAC CTT CGG ATA TAC TGT CAA	501 bp	Grierson et al., 2004 [37]
PCV2	2B	TAC ATG GTT ACA CGG ATA TTG TA	501 bp	Grierson et al., 2004 [37]
HEV	JVHEV-F	GGT GGT TTC TGG GGT GAC	/	Jothikumar et al., 2006 [38]
	JVHEV-R	AGG GGT TGG TTG GAT GAA
	JVHEV-P	6-FAM-TGA TTC TCA GCC CTT CGC-BMQ

ISSN: 1746-6148