Molecular detection of porcine reproductive and respiratory syndrome virus, porcine circovirus 2 and hepatitis E virus in oral fluid compared to their detection in faeces and serum

Table 2 Presence of viruses in OF, faeces and serum on each farm in different pig categories

	Farm	5 w/o	7 w/o	9 w/o	11 w/o	Fatteners	Breeding sows
PRRSV^a
OF	5	neg.	pos.	pos.	pos.	pos.	neg.
faeces		neg.	neg.	neg.	pos.	neg.	neg.
serum pool		neg.	NT	NT	NT	NT	neg.
individual sera^d		NT	10/10	10/10	10/10	0/10	NT
PCV2^b
OF	2	neg.	neg.	pos.	neg.	neg.	neg.
faeces		neg.	neg.	pos.	neg.	neg.	neg.
serum pool		neg.	neg.	neg.	pos.	pos.	neg.
individual sera^d		NT	NT	0/10	8/10	3/10	NT
OF	4	neg.	neg.	neg.	pos.	neg.	neg.
faeces		neg.	neg.	neg.	neg.	neg.	neg.
serum pool		neg.	neg.	pos.	neg.	neg.	neg.
individual sera^d		NT	NT	6/10	0/10	NT	NT
OF	6	pos.	pos.	pos.	neg.	neg.	neg.
faeces		pos.	pos.	pos.	neg.	neg.	neg.
serum pool		NT	NT	NT	neg.	neg.	neg.
individual sera^d		2/10	3/10	9/10	NT	NT	NT
HEV^c
OF	6	pos.	pos.	pos.	neg.	neg.	neg.
faeces		pos.	pos.	pos.	neg.	neg.	neg.
serum pool		NT	NT	NT	neg.	neg.	neg.
individual sera^d		0/10	1/10	2/10	NT	NT	NT

neg. negative result, pos. positive result, NT not tested
^a classic RT-PCR
^b classic PCR
^c real-time RT-PCR
^dten individual sera were tested from each pig age group. Results are shown pos./all tested sera

ISSN: 1746-6148